Powdered Fecal Material for Transplant via Enema (Canine)

Download the AnimalBiome Veterinary FMT via enema protocol here.

FMT via Enema For Domestic Dogs

Procedure based on methods described in Pereira et al.,2018

Follow standard practices prior to surgical procedures.

Materials Needed

• 60ml catheter-tip syringe
• Saline solution (0.9% NaCl)
• Enema packet(s)
• Red rubber catheter (for warm water enema)
• Foley catheter or clean rubber red catheter (for FMT procedure)
• Gloves
• ABV Enema Jar
• Non-spermicidal lube

Step 1: Prepare Patient

Materials

• 60ml catheter-tip syringe
• Red rubber catheter
• Saline solution (0.9% NaCl)
• Gloves

Steps

Note: sedation is recommended, but not required.

1. Place the patient into right lateral recumbency with their pelvis slightly elevated.
2. Administration of warm water enema (recommended).
   1. Draw the appropriate amount of warm water into a 60ml catheter-tip syringe.
   2. Attach the syringe to a lubricated red rubber catheter and aspirate catheter to avoid introducing air into the colon.
   3. Insert the catheter up to the estimated region of the transverse colon.
   4. Administer the warm water enema.
   5. Remove the catheter and allow the evacuation of the colon.

Step 2: Prepare FMT Slurry

Materials

• 60ml catheter-tip syringe
• Red rubber catheter
• ABV Enema packet(s)
• Saline solution (0.9% NaCl)
• ABV enema jar
• Gloves

Steps

1. Using a lidded wide-mouth jar, mix the number of pouches of fecal powder based on patient weight with the calculated volume of 0.9% NaCl (see full dosage chart below). Pour the saline slowly into the fecal powder to minimize powder aerosolization.
2. Secure lid. Mix thoroughly by shaking (1 minute) until a smooth-consistency slurry results.
3. Transfer the fecal slurry into a catheter-tipped syringe(s) for administration to the patient.
4. The fecal slurry should be administered within 2 hours of reconstitution.

Step 3: Administer FMT Materials

Materials

• FMT Slurry (see step 2)
• Foley catheter (or rubber red catheter)
• 60ml catheter-tip syringe with FMT slurry contents
• Gloves

Steps

Note: Spermicidal lubrication should be avoided for this step.

1. Insert the 60ml syringe into the Foley catheter and flush the catheter to avoid introducing air into the colon.
2. Insert the lubricated Foley catheter rectally, up to the distal region of the transverse colon and proximal to the descending colon. Then inflate the catheter to reduce seepage.
3. Administer the desired volume of fecal slurry slowly.
4. Enema Retention
   1. 15 min in right lateral recumbency
   2. 15 min in sternal position
   3. 15 min in left lateral recumbency

Parvo Puppy Protocol

1. Mix (1) 15 g packet with 15 ml of 0.9% sodium chloride solution.
2. Aspirate 15 ml of the fecal slurry into a 20ml syringe and attach a lubricated red rubber catheter.
3. Place patient in right lateral recumbency with pelvis tilted to approximately 45-degrees and advance catheter into the colon.
4. Keep the patient in lateral recumbency for 2 minutes to aid in the movement of the fecal material through the colon.
5. Repeat enema every 24 hours until resolution of diarrhea.

Find dosing chart for dogs on page 3 of this protocol.

Reference:

Pereira, Giorgio Q., Lucas A. Gomes, Iago S. Santos, Alice F. Alfieri, J. S. Weese, and Marcio C. Costa. "Fecal Microbiota Transplantation in Puppies with Canine Parvovirus Infection." Journal of Veterinary Internal Medicine 32.2 (2018): 707-11. Print.

Each jar comes with up to five 15g individually-packed Mylar pouches with prescreened canine fecal microbial transplant sourced from select healthy dog donors who live in homes, rigorously tested for pathogens, parasites, and healthy microbiome composition. Learn more about our screening process here.

This is a natural product. Some variation in color is normal.

Actual number of Mylar pouches per jar varies depending on your order quantity.